Not always. North Downs Tunnel on HS1 has no airshafts for example. I need to get to my archives to be able to tell you why a particular distance was used to determine when something became a tunnel and I cant do that until tomorrow. The European Railway Agency define a tunnel as being 100m long meaning something of that length or greater must have a full Risk Assessment and Evacuation Plan.
Evacuation procedures do, of course, vary with each site. Modern tunnels that comply with the Interoperability TSI will have clear walkways down one side of the train as per North Downs and Heathrow. Others wont and rely on end cab evacuation, East London line for example. Signalling systems are usually designed such that trains are not stopped in tunnels - at least modern systems are! But there are still some places where this happens. It's all down to the Risk Assessment and how the Local Fire Authority view the situation.
What I was referring to was the need for ZHLS cabling which must be provided in a "Tunnel" as opposed to a long bridge. All Tunnels have to have that kind of cable and from memory the length is 500m.