Ah, I see! Certainly not in recent times. The last services terminate at Portsmouth and return to Fratton depot for stabling, and are worked by Fratton crews.
What does still happen is that the last service of the night, having started from Bristol Parkway rather than Cardiff, runs Romsey - Soton Ctl - Soton Airport - Eastleigh (reverses) - Botley (not calling) - Fareham, rather than the usual route straight from Soton Ctl to Fareham via the Netley road, which is for crew route knowledge retention purposes.
The last northbound of the day runs the same route in reverse, terminating at Westbury and manned by a Westbury crew. Oddly, this one northbound service alone runs as a 2Mxx headcode, rather than the 1Fxx headcodes used for the same services throughout the rest of the day. The penultimate northbound service runs normal (Netley) route but terminates at Bristol Temple Meads.